Investigation of the Effect of Bioptron Light in Preventing the Formation of Scars in Diabetic and Non-Diabetic Mice

 The formation of skin scars is inevitable following surgery, trauma and burns.  Wound healing is a complex process that occurs during the competition of collagen production and collapse mechanisms. Various factors such as diabetes can interfere this process with multiple mechanisms. There are also ways to speed up the process of repair and prevent wound formation which is the use of light. The present study examines the effect of polarizing light (bioptron) on the process of wound healing and prevention of wound formation in diabetic and non-diabetic rats.

A total of 52 rats were studied in four groups of 13 with and without diabetes and with and without using light bioptron: First group: without diabetes, without light; group 2: without diabetes, with light; group 3: with diabetes, without light; group 4: with diabetes, with light. At first, the wound was formed about 2 cm in rats and sutured. Then grouping wound was dressed with normal saline daily in two groups with and without diabetes and the other group was exposed to bipotron light at a distance of 10 cm for 10 minutes with a power of 2.4 joule cm2. Then the scars were scanned by using the Stony Brook Scar Scale for objective measurements of the wound over a period of 21 days. In the end, the rats were sacrificed and the wounds were sent to the lab for histological examination. The incidence of wound was studied based on histological examination on necrosis parameters, fibroblastic proliferation, collagen accumulation, granulation tissue maturation, epithelialization and neovascularization.

There was no significant difference between the two groups in terms of SBSES scores on days 7, 14 and 21. There was no difference in histopathological characteristics except for non-diabetic rats that bioptron were reduced fibroblast proliferation(pvalue<0.001) and decreased neovascularization(pvalue<0.001).

Despite the lack of significant differences between groups, using of polarized light can be corrected by reducing the proliferation of fibroblasts in the wound healing and reducing neovascularization to improve the appearance of the scar and can be used as a safe method.