Evaluation of the effect of salicylic acid on the increase of secondary metabolite of Trigonellin in in vitro culture of Trigonella foenum-graecum

Trigonella foenum-graecum is annual plant and dicotyldones of the fabaceae family. Root, leaf and seed have important medicinal compounds. Manipulation of cell culture media with elicitors is one of the important strategies for induction of secondary metabolism and production of valuable metabolites. Salicylic acid as a non-biological elicitor is effective in increasing the production of pharmaceutical metabolites. The aim of this study was to investigate the effect of salicylic acid on growth, some physiological and trigonelline production in in vitro culture of fenugreek. Fenugreek cell culture was obtained using cuticle extract of TN-47-155 fenugreek genotype in MS medium containing 0.35 mg / l TDZ and 0.05 mg / l IBA. Salicylic acid at concentrations of 12.5, 25, 50 mg / L were used. The cells were then treated with triglyceride and other physiological parameters after one week of treatment. The results of HPLC showed that cell growth and viability of the cells decreased as compared to the control. The amount of hydrogen peroxide and membrane lipid peroxidation in the cells increased compared to the control (without hormone) treatment. All treatments increased the production of TG and total phenol. Treatment of cells with 50 mg / l (75%) salicylic acid increased triglyceride levels twice as much as control (35%). Salicylic acid can be used as a stimulant in fenugreek cell culture and induces higher triglyceride production.