Polymorphism of Vascular Endothelial Growth Factor Gene−1154 G/A in Women with Recurrent Miscarriage and IVF Failure; A case–control study

Vascular endothelial growth factor A (VEGFA) plays a key role in angiogenesis during human placenta formation and its abnormal expressions have been reported in placental tissues of women with recurrent miscarriage (RM).

The aim of this study was to evaluate the correlation of polymorphism of VEGF1154 G/A gene in RM and In Vitro Fertilization (IVF) failures.

The peripheral blood samples of women with RM, IVF failures and healthy women with live born children, as control group were collected. DNA samples were isolated and VEGF 1154G/A polymorphism was evaluated by polymerase chain reaction and restriction fragment length polymorphism. Single nucleotide polymorphism scanning was done using MnII restriction enzymes for 1154 G/A.

The findings of this study showed that the VEGF 1154 A/A and VEGF 1154 G/A mutation frequencies in both RM and IVF failure groups were significantly higher than those in the control group (p=0.005). The homozygous AA mutant genotype frequency in the control group was 0, While in the RM and IVF failure groups it was 30% and 13.9%, respectively (p=0.005). Moreover, the heterozygous AG genotype frequencies were higher in the RM (66.7%) and IVF failure (77.8%) compared to those in the control group (58.3%).

It was concluded that VEGF 1154 A/A and VEGF 1154 G/A polymorphisms were associated with both RM and IVF failures. However, their relation with IVF failures was more common than RM in A/G genotype, while in A/A, the RM was higher than IVF failure.