Today, microscopic studies of soft samples, such as polymers and life science, are one of the most important issues for sample preparation. Generally, in the preparation of cross sections of samples, common methods such as microtome, ultra microtome and fracture in liquid nitrogen are used. Often, the cuttings resulting from these methods have unintended defects and artifacts, including knife rejection on the sample, soft sample crushing, inappropriate cutting due to the angle between the blade and sample, the effects of machine shake or Knives, the effects of thermal changes during work, shrinkage on the samples, the composition of the resulting layers and the difficulty of separating them, and in addition, it is very time-consuming. Due to the fact that FIB-SEM technique is used as a very powerful technique for sample preparation, inspection and processing of equipment and machining in various fields, in the preparation of samples The above can easily be replaced by minimizing the unwanted effects of conventional methods. Nowadays, in order to improve the efficiency, it is possible to investigate three-dimensional structures on the micro and nano scale, the possibility of studying on the situ, the possibility of preparing sequential films and the ability to create images and control all the stages of preparation and machining of samples. The FIB-SEM technic is very useful. Therefore, in this paper, some of the applications have been studied in order to become familiar with the function of this technique in the life sciences and polymers.
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