Effects of Hydroalcoholic Leaf Extract of Avicennia marina on Apoptotic, Inflammatory, Oxidative Stress, and Lipid Peroxidation Indices and Liver Histology of Type 1 Diabetic Rats
Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background

Avicennia marina (A. marina) has been traditionally used in the treatment of various diseases due to its antiinflammatory and antioxidant properties.

Objectives

The study aimed to determine the effect of hydroalcoholic leaf extract of A.marinaon apoptotic, inflammatory, oxidative stress, and lipid peroxidation indices and liver histology of type 1 diabetic rats.

Methods

In this laboratory research, a total of 40 male Wistar rats were randomly divided into five groups including the control group, type 1 diabetes mellitus (DM) group, diabetic group receiving 50 mg/kg of hydroalcoholic extract of A. marina, diabetic group receiving 100 mg/kg of A. marina, and the diabetic group receiving 200 mg/kg of A. marina. The control and DM groups were intraperitoneally administered with 0.5 mL of saline solution as the solvent for 30 days. The experimental groups were intraperitoneally administered with 0.5 mL of hydroalcoholic extract at doses of 50, 100, and 200 mg/kg for 30 days, respectively. Diabetes mellitus was induced by an intraperitoneal injection of 120 mg/kg of alloxan monohydrate. At the end of treatment, we measured the serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6. Also, we assessed the levels of BCL2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), caspase-9, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), malondialdehyde (MDA), and 8-hydroxy-2’-deoxyguanosine (HOdG-8) using ELISA in liver tissues. Histological changes in the liver were also investigated.

Results

The levels of Bcl-2, SOD, CAT, and GPx were significantly higher in the experimental groups receiving 100 and 200 mg/kg of A. marina in a dose-dependent manner than in the DM group. The levels of Bax, caspase-9, MDA, and HOdG-8 were significantly lower in the experimental groups in a dose-dependent manner. Also, the serum levels of TNF-α, IL-1β, and IL-6 were significantly lower in these groups in a dose-dependent manner (P < 0.05). However, the differences were not significant in the experimental group receiving 50 mg/kg of A. marina (P > 0.05). The results showed that A. marina extract improved the histological changes in the liver of diabetic rats in a dose-dependent manner.

Conclusions

The administration of the hydroalcoholic extract of A. marina can improve liver tissue damage and reduce diabetesrelated liver complications by decreasing apoptosis, inflammation, oxidative stress, and lipid peroxidation.

Language:
English
Published:
Hepatitis Monthly, Volume:20 Issue: 4, Apr 2020
Page:
5
magiran.com/p2135504  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!