Antioxidants are free radical scavengers, which protect the body against oxidative damages. Marine organism have also attracted attention in research on natural antioxidants. Therefore antioxidative properties of ascidian (Phallusia nigra) extracts from Oman Sea were investigated. DPPH free radical scavenging, reduction power and metal chelating activity were employed to identify the antioxidant properties by using methanol, ethanol and chloroform solvents. Experimental results of this study were presented as mean ± standard deviation of three replications and for analyzing data were analyzed by one-way ANOVA and Duncan multiple range test. The concentration of (1 mg/ml) methanol gained the highest percentage of DPPH radical scavenging (i.e. 93.09 ± 1.5 %). In reduction power assay, concentration of (1 mg/ml) methanol (0.35 ± 0.01) was higher in comparison with other extracts. In all the concentrations, Metal chelating activity of chloroform extract was reported to have the highest percentage. In all three assays, there was a direct relationship between concentration, control and absorption power of extract. In this study, the antioxidant properties of Phallusia nigra showed to be significant and could be used for further studies in order to find new pharmaceutical compounds for treating many diseases like cancer. Moreover After preclinical and clinical studies, the extract can be used in cosmetic and veterinary medicine industries.
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