This study aimed to investigate the effect of five herbicides (Tribenuron-methyl, Metribuzin, Trifluoralin, Haloxyfop-methyl, and 2, 4-D (on the soil dehydrogenase and urease activities.
The experiment was conducted as a completely randomized design with five herbicides and a control with three replicates for six months and the enzyme activities were measured at beginning of the experiment, and after three and six months. The concentration of herbicides was 0.27, 0.67, 0.67, 0.67, and 0.1 mg/kg soil for Haloxyfop-methyl, Metribuzin, Trifluoralin, 2, 4-D, and Tribenuron-methyl, respectively.
The results showed that the effect of herbicides on the enzyme activities was significantly different (p0.01). After 3 months, the herbicides decreased the urease activity in comparison to the control (34.9 µgN.g-1). The most reduction was related to Trifluoralin (24.8 µgN.g-1) which was 28.9% lower than the control. Urease activity increased after six months compared to three months, and this increase was 41.5% for Trifluoralin herbicides (35.1 µgN.g-1). Urease activity in control treatment in six months was not significant compared to the three months. All of the herbicides significantly reduced the dehydrogenase activity after three months compared to the control (0.6 µgTPF.g-1), so that the dehydrogenase activity was observed in Haloxyfop treatment with 0.01 µgTPF.g-1, which was 98% lower than the control. In all treatments, dehydrogenase activity after six months was significantly higher than that in the three months, so that the highest dehydrogenase activity was observed in 2,4-D treatment (1.44 µgTPF.g-1), which was significantly higher than the control.
The results show that the herbicides significantly decreased the activity of the enzymes compared to the control during the three months. After six months the enzyme activities recovered to the level before herbicides application. Probably this was due to a short lifetime of herbicide in the soil which was less than six months.
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