Evaluation of Cytochrome P450 Gene Expression as Physiological Pollution Biomarkers in Broiler Chickens Fed Silver Nanoparticles

Nowadays, researchers are converted silver to nanometer dimensions and using nanotechnology to achieve suitable performance and preserve the poultry health and they are used silver nanoparticles are used for poultry nutrition as a nutritional supplement. On the other hand, at the molecular level no complete reports in poultry breeding have been provided.

This study was conducted to evaluate cytochrome P450 gene expression in broiler chickens fed with silver nanoparticles.

This experiment a completely randomized design with 450 single-day Cobb 500 broiler chicks was divided into five treatments and six replications (15 birds in each replicate). Chickens were fed with (1) control (basal diet), (2) basal diet containing 1% zeolite, (3) basal diet containing 1% of zeolite-coated with 0.5% silver nanoparticles, (4) Basal diet containing with 0.15% organic acids and (5) Basal diet containing 1% of zeolite-coated with 0.5% of silver nanoparticles and 0.15% organic acids in with or without heat stress condition.

Results demonstrated the level of expression of cytochrome P450 in broilers fed zeolite (Z) in the 21st day of experiment, silver nanoparticles coated with zeolite in combination with organic acid (NSOA) in 42nd day without heat stress condition and silver nanoparticles coated with zeolite (NS) in day 42 with heat stress condition in intestine and liver had significant increase in expression in comparison with control (C) treatment (P<0.05).

In conclusion, result showed that zeolite and silver nanoparticles were identified as inorganic, chemical and exogenous substances in liver and intestine and up-regulated expression of pollution biomarkers genes. Whereas this effect is not identified in organic acid.