Investigating the rate of pouring of beef produced in Zahedan to Escherichia Coli using MPN and detection of O157:H7 by PCR

Beef contamination usually occurs in the killing process. One of the intestinal bacterial pathogen that causes disease in human and animals everywhere is bacterial pathogen E. coli. One of the important serotypes of this bacterium is E. coli O157: H7. The aim of this study was to determine the rate of contamination of beef produced in Zahedan to E. coli by MPN Method and also O157:H7 strains isolated by PCR. During this study, conducted within six months from July 1396 to July 1397, 360 beef samples from cattle meat packages of Zahedan City, from different parts of the carcasses isolates of the heat-resistant E. coli in the laboratory was carried out using the MPN method. Seventy isolates were finally detected. The final test was performed using a specific primer as rfb to detect E. coli O157:H7 using a PCR reaction. Only one isolate was confirmed based on this serotype, which indicates the proper health status of the meat produced in Zahedan. In the present study, a molecular study of 2 isolates of heat-resistant Escherichia coli bacteria was done. In the study of rfb gene, only one isolate in terms of Escherichia coli serotype 7H: 157O was confirmed. With this study, the presence of this bacterium in beef samples is confirmed, which can indicate that the presence of this bacterium in other raw animal products in this province should also be investigated and tracked.