The Effect of High Intensity Interval Training Preconditioning on Klotho and TNF-α Female Mice with Multiple Sclerosis

 Exercise activity plays an important role in increasing biomarkers such as Klotho and PLP and improves clinical  outcome in mice with MS. In the present study, we evaluated whether 6 weeks of high-intensity interval training (HIIT) increases Klotho or PLP levels and decreased TNF-α in cerebellum tissue.  

Animals were divided into two groups of training (EX) and control (Con). After 4 weeks, the exercise and control groups were divided into two groups and EAE was induced in half of the animals in the control and EX groups. In the EX-6 weeks group after 4 weeks of training, EAE induction and training protocol continued until the sixth week. In the EX-4 weeks group after 4 weeks of training, EAE was induced and the training was stopped.

Klotho and PLP levels increased in the EAE-EX1 group compared to the EAE and EAE-EX2 groups (P≤0.0001). Also, demyelination levels decreased in the EAE-EX1 group compared to the EAE group (P≤0.007). TNF-α concentration decreased in both EAE-exercise groups (P≤0.0001).

The results of this study showed that HIIT increased Klotho and PLP levels and improved clinical outcomes in the in mice with MS.