Phylogenetic typing of Escherichia coli isolates collected from broilers with Collibacillosis in Zabol

Escherichia coli is an opportunistic organism which can cause Colibacillosis syndromes (colisepticemia, pericarditis, perihepathitis, salpingitis and …). E.coli has four phylogenetic groups including A, B1, B2 and D. Phylotyping is an adequate method for surveying the dissipation type of the phylogenetic groups of E.coli isolates in different regions. The present study was conducted to determine the phylogenetic groups of E.coli in Zabol city. In order to determine the frequency of the phylogenetic groups of E.coli in Zabol, 144 broilers suspected of Colibacillosis were sampled and samples were transferred to laboratory in TSB. After culturing and applying some common biochemistry tests, a total number of 100 E.coli were isolated. DNA of all isolates was extracted by boiling method and phylogenetic groups were determined by triplex PCR procedure. In this study; 36 %, 27 %, 23 % and 14 % of 100 E.coli isolates belonged to B1, D, A and B2 phylogenetic groups; respectively. The present study concluded that almost all of E.coli samples which were collected from broilers suspected of Colibacillosis in Zabol belonged to B1 phylogenetic group.