Investigating the Protective Role of N-Acetyl Cysteine and S-Methyl Cysteine in the Activity of Antioxidant Enzymes and the Level of lipid Peroxidation Due to Acetamiprid Administration in Rat Kidney and Serum
Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background & Objective

Acetamiprid (ACP), as a neonicotinoid toxin, causes free radicals production and oxidative stress in various organisms. The aim of this study was to evaluate the antioxidative effects of N-acetylcysteine (NAC) and S-methylcysteine (SMC) on reducing acetaminoprid-induced oxidative stress in serum and kidney of rats.

Materials & Methods

In this experimental study, 42 male Wistar rats were randomly divided into 6 groups of 7 including one control, one sham (normal saline) and five experimental groups, which intra-peritoneally received ACP (5 mg/kg), NAG (160 mg/kg), SMC (100 mg/kg), ACP+NAC, ACP+SMC, and ACP+ NAC+SMC for one week. After separation of serum and kidney tissue, the activity of the catalase (CAT), glutathione S-transferase (GST), glutathione concentration (GSH), malondialdehyde (MDA) and total antioxidant (TAC) was determined.

Results

Acetamiprid caused significant increase in GST activity in serum and kidney (p< 0.01), CAT activity in serum and kidney (p< 0.05) but insignificant increase in MDA level and insignificant decrease in GSH and TAC compared to control. NAC and SMC, alone and in combination with ACP, restored the levels of TAC, GSH and MDA and activities of CAT and GST.

Conclusion

Acetamiprid increases lipid peroxidation, activity of CAT and GST, and decreases the concentration of GSH and TAC, presumably by producing free radicals. Administration of NAC and SMS as antioxidants causes a decrease in acetamiprid toxicity due to relative reduction of free radicals.

Language:
Persian
Published:
Journal of Advanced Biomedical Sciences, Volume:10 Issue: 1, 2020
Pages:
2081 to 2093
magiran.com/p2146235  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!