The Effect of Gaillardin on Proliferation and Apoptosis of Acute Lymphoblastic Leukemia Cell Line (Nalm-6)
Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background

Due to increased cancer chemotherapy resistance, the use of natural compounds in cancer treatment has been considered in recent years. Inula-Oculus-Christi, is one of the high importance plants in the field of traditional medicine. It has been shown that extract of this plant has cytotoxic effects on breast cancer cells.

Objective

Therefore, the aim of this study was to evaluate the cytotoxic effect of Gaillardin and apoptosis induced by this extract on acute lymphoblastic leukemia cells.

Methods

Gaillardin from Inula-Oculus-Christi plant was extracted by Traditional Medicine Research Center of Shahid Beheshti University of Medical Sciences. The cytotoxic effect of Gaillardin on the acute lymphoblastic leukemia (Nalm-6) and PBMC (as a normal cell line) was investigated at different doses for 24 and 48 hours. In this study, Trypan blue, MTT assay and DAPI staining were used. In addition, Annexin V/PI staining was utilized for confirming apoptosis.

Results

The results of Trypan blue and MTT assay demonstrated the induction of apoptosis and reduction in proliferation of Nalm-6 cells treated by Gaillardin, showing a time and dose-dependent manner (P < 0.001). However, low sensitivity to the different concentrations of Gaillardin was observed in PBMCs. DAPI staining and Flow cytometry analysis confirmed a significantly high percentage of apoptotic cells compared with control groups (P < 0.001).

Conclusion

According to the results, Gaillardin demonstrated cytotoxic effects on Nalm-6 cells, but

Language:
Persian
Published:
Journal of Medicinal Plants, Volume:19 Issue: 74, 2020
Pages:
108 to 117
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