Investigation of the direct regeneration from node explant of Capparis spinosa L. in MS medium containing different hormones

Caper bush (Capparis spinosa L.), is a perennial medicinal plant of hot and dry climates that grows in the summer. This plant contains important compounds such as flavonoids, terpenes, alkaloids, glycosides, and glycosinolates. Despite the ever increasing need for the mass reproduction of this plant, there is little information about the methods of its proliferation. In this research, the cotyledon leaf, leaf, bud, stamen, cotyledon upper axis, root, petal, sepal, and node explants were cultured in MS medium containing KIN, NAA, BAP and IBA hormones at different concentrations in order to direct regeneration of seedlings for shoot. Experiment was conducted to identify the best media for production of shoots and roots in vitro cultivation of caper different explants. In present experiment, except node explants, none of the other explants were regenerated, and the regeneration from node explants were achieved in 5 to 8 weeks, where MS medium containing 2 mg/L BAP and MS medium containing 1 mg/L NAA were the best medium for production of shoots and rooting, respectively. Considering the importance of Capparis spinose as medicinal plant and its difficulty to reproduction by seed, use of these media and node explant are recommended to production of this medicinal plant under in vitro culture conditions.