Optimization of polyhydroxybutyrate production with novel strain bacillus cereus separated from petrochemical wastewater

Polyhydroxybutyrate is a biopolymer Produced by bacteria. Also, with having chemical and physical characteristics similar with artificial plastics are completely biodegradable and compatible with life-environment. This study has been done with the purpose of separating and knowing one local strain with the ability of high-production for industrial purposes.

In the present analytical research, the sampling from petrochemical wastewater has been done. The existence of polyhydroxybutyrate in separations has been studied with Sudan black staining. One separation of bacillus was chosen to increase the production of polyhydroxybutyrate. This separation was distinguished with biochemical methods and determining 16S rRNA gene sequencing. The final confirmation of Polyhydroxybutyrate synthesis was done through Fourier transform infrared spectroscopy and Proton nuclear magnetic resonance. To increase more production of polyhydroxybutyrate, the effect of different factors including carbone, nitrogen, pH and temperature were assessed.

As a whole, eight bacterial isolation producing polyhydroxybutyrate were separated that among them the one novel strain of bacillus cereus was chosen as better separation. The best conditions to increase more production of polyhydroxybutyrate, was the application and use of glucose as carbon source, ammonium sulphate as nitrogen source, 7 pH and 30°C temperature.

Valuable data on optimized conditions for PHB production has been obtained through the present research that can be used at industrial level for PHB production, so it can be called a quick appearing alternation for petroleum based on synthetic plastic.