Evaluation of the presence of Subtilisin (SUB) gene activity in Trichophyton verrucosum and Microsporum gypseum in human, animal and soil samples with PCR method and Dendrogram designing

Subtilisin genes, which encoding serine protease, have an important role in the pathogenicity of dermatophytes. The aim of this study was considered the presence of Subtilisin gene (SUB) and its similarity in 25 clinical and nonclinical samples of two dermatophytes, Trichophyton verrucosum (T. verrucosum) and Microsporum gypseum (M. gypseum), by using molecular method and designing dendrogram.64% (16/25) strains showed the active presence of the gene. Statistical analysis of data showed the correlation among the presence of SUB gene, the host (human/ animal/ soil) and the type of dermatophytes (p0.05).By using NTSYS software, UPGMA method and cut off number 70%, the strains were classified in 10 different genotypes according to their similarities to SUB gene (the Simpson coefficient of primers was 0/88).Due to different reports of presence/absence of Subtilisin gene in clinical and nonclinical strains of Trichophyton verrucosum and Microsporum gypsum,in this study, new primers were designed and synthesized to confirm the presence of Subtilisin gene in strains 64% of the, which, of course, requires more studies for further confirmation.