The Effect of Intake Duration of Curcumin Supplementation During Strenuous Endurance Training on GPX Activity and MDA Levels of Liver, Heart and Skeletal Muscle in Male Wistar Rats

The purpose of this study was to investigate the effect of intake duration of curcumin supplementation on lipid peroxidation and antioxidant capacity of liver, heart and skeletal muscle during eight weeks of strenuous endurance training in male wistar rats. In this experimental study, 39 male wistar rats (age= 8 weeks; weight= 226.76±18.64 g) after one week familiarization period, were randomly divided in to six group; Control, Curcumin, 48hrs Curcumin, Endurance, Endurance+Curcumin and Endurance+48hrs Curcumin. Endurance training (eight week, five sessions a week) carried out on rodent treadmill. Rats received curcumin supplement 30 mg/kg.BW by sub peritoneal injection (eight weeks, three sessions) or (last 48 hours, every 8 hours). GPX activity were measured using ELISA kits and MDA levels measured by spectrophotometric method. One-way ANOVA results showed that GPX activity of liver (P=0.001) and heart (P=0.034) were significantly lower, and MDA levels of liver (P=0.001) and heart (P=0.004) were significantly higher in endurance group in compared with control group. Also, GPX activity of liver in endurance+curcumin (P=0.002) and endurance+48hrs curcumin (P=0.001) groups were significantly higher than endurance group. MDA levels of liver (p=0/007) and heart (p=0/018) in endurance+curcumin group were significantly lower than endurance group and MDA level of heart in curcumin group was significantly higher than control. There were no significant differences between GPX and MDA activity of skeletal muscle. Based on our results, strenuous endurance training induced different oxidative stress responses in different tissues. On the other hand, curcumin supplementation during 8 weeks and even last 48 hours of strenuous endurance training, prevents oxidative stress and improves antioxidant capacity in liver and heart.