Comparison of the effects of two types of high intensity interval training on the gene expression of collagen 1, 2, and SMAD/3 in the left ventricle of male rats in type 2 diabetes induced by streptozotocin
Message:
Abstract:
Background and Objective

High-intensity interval training reduces diabetic cardiomyopathy by regulating gene expression in patients with diabetes. The aim of this study was to compare the effect of two types of high intensity interval training on the expression of collagen 1, 2 and SMAD/3 genes in the left ventricle of male rats with type 2 diabetes.

Materials and Methods

The present study was experimental. For this purpose, 28 male Wistar rats were divided into four groups: type 1 high-intensity interval training (HIIT1:1), type 2 high-intensity interval training (HIIT2:1), diabetic control (DC), and non-diabetic control (NC). Diabetes was induced in all groups except non-diabetic control group by intraperitoneal injection of streptozotocin (STZ) after 12 hours of overnight fasting. 24 hours after the last training and recovery session, their left ventricle was extracted. Plasma glucose concentration was measured by the glucose oxidase method, ELISA method was used to measure the insulin levels, and the HOMA-IR method was used to measure the insulin resistance index. A real-time PCR technique was used to evaluate the gene expression of the collagens 1 , 2 , and SMAD/3 genes. Results were analyzed with one-way ANOVA and Tukey post hoc tests.

Results

Collagen 1 did not show a significant difference between the two exercise groups (p = 0.112). The mean values of both exercise groups were significantly lower than in the diabetic control group in collagen expression (p = 0.0001). The mean values of collagen 2 gene expression in the HIIT2: 1 training group were significantly lower than the HIIT1: 1 group (P = 0.044) and diabetic control (P = 0.002). The mean values of SMAD / 3 gene expression in HIIT2: 1 group were significantly lower than HIIT1: 1 (P = 0.002) and diabetic control (P = 0.020). The mean weight values and insulin resistance index in the HIIT1 group and glucose in the HIIT2: 1 group were significantly lower.

Conclusion

Based on the findings, it can be concluded that 4 weeks of type 2 HIIT compared to other groups had a higher effect on reducing the expression of collagen 2 and SMAD / 3 genes in the left ventricle of diabetic rats with down-regulated of genes. Therefore HIIT-2  may improve diabetic cardiomyopathy syptoms.

Article Type:
Research/Original Article
Language:
Persian
Published:
Daneshvar Medicine, Volume:28 Issue: 148, 2020
Pages:
42 - 54
magiran.com/p2164927  
برخی از خدمات از جمله دانلود متن مقالات تنها به مشترکان مگیران ارایه می‌گردد. شما می‌توانید به یکی از روش‌های زیر مشترک شوید:
اشتراک شخصی
در سایت عضو شوید و هزینه اشتراک یک‌ساله سایت به مبلغ 400,000ريال را پرداخت کنید. همزمان با برقراری دوره اشتراک بسته دانلود 100 مطلب نیز برای شما فعال خواهد شد!
پرداخت با کارتهای اعتباری بین المللی از طریق PayPal امکانپذیر است.
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی همه کاربران به متن مطالب خریداری نمایند!
توجه!
  • دسترسی به متن مقالات این پایگاه در قالب ارایه خدمات کتابخانه دیجیتال و با دریافت حق عضویت صورت می‌گیرد و مگیران بهایی برای هر مقاله تعیین نکرده و وجهی بابت آن دریافت نمی‌کند.
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.