Antioxidant effect of carob seedextract (Ceratoniasiliqua L) on quality parameters Farahani ram sperm after freeze-thawing

The aim of this study was to determine the effect of Carob seedextract as natural antioxidant on quality cryopreserved Farahani rams breeding sperm.

In this study semen was collected from five mature ram (weight: 60±5 kg) twice a week using an artificial vagina and the ejaculates were pooled in order to eliminate the individual effect of rams. Different levels of carob seed extracts (0, 0/05, 0/1, 0/15 and 0/2 mL) were added to diluent based tris-egg yolk. After cooling, filling and sealing of the samples, they were frozen and with nitrogen vapor and immersed in liquid nitrogen and were stored until evaluation time. Thereafter and after thawing and incubation for5 min, sperm quality parameters includingmotility, progressive motility, viability (Nigrosine–eosin staining), membrane integrity with Hypoosmotic (Host) and morphology abnormality (Hancock test).

Results indicated that the level of 0/05 mL carob seedextract significantly improved some parameters including motility, viability, plasma membrane and morphology integrity compared to control (P<0.01).

Results indicated that so added 0/05 of carob seed extract peel to Tris based extender was beneficial in storage in sperm Farahani ram breeding after freeze-thawing.