Evaluation of Antifungal Effects of Prangos ferulace and Plantago major L Plants Against Fluconazole-resistant Candida albicans Species in Extracorporeal Conditions

Medicinal plants can be effective in treating diseases due to having various antifungal compounds. The aim of this study was to evaluate the antifungal effects of Prangos ferulace and Plantago majorL against fluconazole-resistant Candida albicans in extracorporeal conditions.

This study was experimental. Prangos ferulace and Plantago majorL plants were prepared from stores in Yasuj, Iran, in the spring and then scientifically approved by the Zardband Pharmaceutical Company in Yasuj. The extract was extracted after sending the plants to the Research Center for Medicinal Plants of Yasuj University of Medical Sciences. In order to prepare the aqueous extract of the plants, 3 g of plant powder was added to 30 ml of distilled water and placed on a shaker (Behdad, Tehran, Iran) for 24 h. The strain used in the present study was Candida albicans ATCC 10231, which was prepared by Shiraz University of Medical Sciences, Shiraz, Iran. In the present study, the alcoholic extracts of Prangos ferulace and Plantago major L were used at the concentrations of 7.5, 15, and 30 mg/ml. The non-growth halo diameter (in millimeters) was measured using the standard paper disc diffusion method. The minimum inhibitory concentrations (MICs) required to inhibit the growth of 90% and 50% of organisms were also evaluated. Finally, the number of living cells was counted, and the data were analyzed using ANOVA test in SPSS software (version 23).

The results showed that at a concentration of 30 mg/ml, the maximum diameters of the non-growth halo were 15.3 mm for Prangos ferulace and 12.4 mm for Plantago major L. Regarding the MIC value, the MIC50 and MIC90 were respectively obtained as 19 and 38 μg/ml for Prangos ferulace and 14 and 29 μg/ml for Plantago major L (p < 0.05). Considering the live cell count, the lowest number of live cells was formed at a dilution of 10. The mean value of this variable was obtained as 7.3 and 4.2 CFU/ml for Prangos ferulace and Plantago major, respectively.

Prangos ferulace and Plantago major L. prevented the activity of Candida albicans due to their effective medicinal compounds.