Molecular Study of BlaIMP and BlaVIM Genes in Pseudomonas Aeruginosa Strains, Producer of Metallo Beta Lactamases Isolated from Clinical Samples in Hospitals and Medical Centers of Tabriz

Pseudomonas aeruginosa is an inpatient opportunistic pathogen that uses a variety of antibiotics, including beta-lactam, to treat infections. The aim of this study was to investigate the abundance of blaIMP and blaVIM genes in P. aeruginosa strains, a producer of metallo beta lactamases isolated from clinical samples in hospitals and medical centers in Tabriz.

In this cross-sectional descriptive study, 100 isolates P. aeruginosa isolated from different clinical samples of patients referred to hospitals and medical centers in Tabriz, collected and after determining phenotypic identity and antibiogram testing, phenotype metallo beta lactamases by CDDT method using imipenem and EDTA/Imipenem disk was evaluated for the bacterium P. aeruginosa.

Out of a total of 100 isolates of P. aeruginosa, 87 samples resistant to imipenem were observed, of which 51 samples were produced by metallo beta lactamases. All metallo beta lactamases had 100% resistance to the antibiotic carbenicillin, meropenem, imipenem, ciprofloxacin, cefotaxime, tobramycin, ceftazidime, amikacin, aztronam. Using the PCR method, it was found that out of 51 samples producing metallo beta lactamases, 12 samples contained blaIMP gene and no positive sample was observed for blaVIM gene.
Discussion and

The results show that most of the samples are drugresistant, although the blaIMP gene did not have a significant frequency, but the abundance of metallo beta lactamases indicates that more care should be taken in prescribing antibiotics for treatment to increase antibiotic-resistant strains.