Effects of trichostatin A on pre- and anti-inflammatory cytokines of the hippocampus in rats of the prenatally restraint stressed model

Prenatal Restraint stress (PRS) leads to cognitive-behavioral disorders and neuro-pathological deficits in children. The present study evaluated the effect of trichostatin A (TSA) on hippocampal level of pro- and anti-inflammatory cytokines in rat model of prenatal restraint stress.

In this experimental study, 20 pregnant Wistar rats were randomly divided into 4 groups, including control, PRS+NS, PRS+TSA5, and PRS+TSA10. PRS was induced from 13 embryonic day, one hour per day during seven days. Two hours before PRS, administration of TSA was intraperitoneally performed. At the end of one month, the hippocampal levels of pro-inflammatory cytokines such as interleukine-1β (IL-1β), interleukine-6 (IL-6),tumor necrosis factor-α (TNF-α) and anti-inflammatory cytokine (interleukine-10; IL-10) were measured by ELISA technique. Statistical differences between groups were analyzed by ANOVA and Tukey post hoc test and p˂0.05 was considered statistically significant.

A significant increase in pro-inflammatory cytokines content and decrease level of IL-10 in hippocampus were observed in PRS+NS group compared to the control (p˂0.05). However, TSA treatment was significantly decreased IL-1β, IL-6 and TNF-α and increased IL-10 compared to the PRS+NS group in dose manner (p˂0.05).

It seems that TSA with anti-inflammatory effects leads to the modification of hippocampal inflammatory cytokines and reduces inflammation in offspring’s hippocampus who exposed to stress in the prenatal period.