Study doubling time, Specific Growth Rate, and multiplication ratio of the Baby Hamsters Kidney 's (BHK) by Altering the Amount of Nutritional Supplementation in the Culture using Taguchi Method

Cell culture is one of the most commonly used techniques in the production of biological products. Many physical and chemical parameters factors may affect cell growth and proliferation. This research (cell culture) has been conducted under conditions of suspension culture. To optimize the nutritional supplements available in the culture medium, different methods are used, such as factor, which takes a long time and has a high error. This study Using the experimental design and Taguchi method, which is more accurate, shorter, and has fewer tests than other methods. . Using this method of experimental design, an attempt was made to optimize the culture medium used in the research. According to the number of final cells, multiplication factor, doubling time and the specific growth rate of each experiment were calculated, and the importance of each of the supplements Food and its effect on the baby hamsters kidney's (BHK) has been considered. The higher the number of ultimate cells, the more time it doubles and the special rate of growth increases, which results in all of these causes cell cultures More productive. The amount of serum added in the culture medium in this method (10%) has the most positive effect on the growth rate, doubling time and specific growth rate. Also, increasing the amount of amino acids (12 gr/li) has no effect on the growth rate, doubling time and specific growth rate.