Highly Pathogenic Avian influenza (AI) is a serious infectious disease caused by negative sense single stranded RNA viruses that belong to the genus Alphainfluenzavirus in the family Orthomyxoviridae. The type A viruses are classified into subtypes based on their surface glycoprotein antigens including hemagglutinin (HA) and neuraminidase (NA). Highly pathogenic avian influenza (HPAI) virus of the H5N1 subtype has spread through domestic poultry or wild birds in more than 60 countries. This article reports heterologous expression in Sf9 insect cells of H5-Hemagglutinin derived from avian influenza virus A/chicken/Iran/53-3/2008(H5N1). The HA nucleotide sequence of an Iranian strain of H5N1 virus from clade 2.2, (A/chicken/Iran/53- 3/2008), was obtained from Genbank. The nucleotide sequence data was codon optimized for insect cells. Using pIEx-3 vector a molecular construct, encoding a secretion signal peptide of adipokinetic hormone (AKH) and 6xHis-Tag coding sequence, upstream of the cloning site for expressing fusion recombinant HA protein with N-terminal tags, were designed and ordered for synthesis by GenScript, Recombinant H5-Hemagglutinin was expressed in Sf9 insect cells as a 95kDa secreted protein. This insect-based Baculovirus-Free heterologous expression system provided functionally recombinant H5-Hemagglutinin that should be useful in anti-influenza drug screening, detection, and diagnostic tests and also as a potential protein-based vaccine.
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.