A study on the abundance and the role of integron class 1 of Escherichia coli isolated from poultry in the induction of bacterial resistance to sodium hypochlorite

The regular use of disinfectants in broiler farms is one of the main bases of the biosecurity. Disinfectants destroy the different disease agents that remained from the last breeding season. Hypochlorite is one of the most suitable disinfectants used in the poultry industry. Nowadays, resistant pathogens to disinfectants and antibiotics are growing worldwide, becoming a serious concern in the public health. Resistance factors are coded by different genes that are commonly present in the different gene cassettes like Integron1 that distribute resistance genes among bacteria. In the previous studies, the relationship between Integron1 and multidrug resistance genes also quaternary ammonium compounds (QACs) resistance genes was clarified. Still, there is no study on the relationship of Integron1 and other disinfectants like Sodium hypochlorite resistance genes. Escherichia coli (E. coli) is one of the most important pathogens of poultry industry. In the present study, 100 isolates of E. coli from the cloak of healthy broilers and from pericarditis cases were studied. Presence of Integron1 gene cassette and orfF gene by Multiplex PCR was detected. Also, the amount of resistance to the Sodium hypochlorite by MIC method was measured. The results showed that there are no significant relationships between the Presence of Integron1 gene cassette and the resistance of the Sodium hypochlorite disinfectant (P<0.05). This result indicates that the genetic reserve and translocation of the resistance gene of Sodium hypochlorite are not related to the Integron1 gene cassette, which shows the need for more studies.