Enterotoxin gene profiles among multi-drug resistance Staphylococcus aureus isolated from traditional dairy products in Fars providence
Staphylococcus aureus is one of the most importantfactors of food poisoning in the world. The objective of this research is to investigate the frequency amount of enterotoxin genes in traditional dairy products with multidrug resistance isolates in Fars province.
This cross-sectional descriptive study was performed on 300 samples of traditional dairy products. In order to identify Staphylococcus aureus isolates in addition to standard biochemical tests, according to PCR method, nuc and 23S rRNA genes proliferation were confirmed. Enterotoxin genes were identified by using of the multiple PCR method. The susceptibility of the isolates for 16 antibiotics was investigated by using of the disk diffusion method according to CLSI criteria.
120 isolates (40%) were identified as Staphylococcus aureus. The highest frequency of contamination is related to Cheese with 38 samples (31.66%) and its lowest relates to Doogh with 10 samples (8.33%). 91 samples (75.83%) of contaminated isolates were included of the enterotoxin gene, the highest frequency of enterotoxin gene was related to sea (54.17%) and its following, seb, seg, see, sed and sec respectively were included the frequency 50%, 33.33%. 28%, 16.67%, 4.17% and 3.50%. Also out of 64 strains of Staphylococcus aureus (56.33%) with multidrug resistance, 54 isolates (84.38%) had enterotoxin genes.
The finding indicated that a significant frequency of Staphylococcus aureus isolates with enterotoxin producing multidrug resistance. Therefore, continuous molecular monitoring of Staphylococcus aureus isolates and also evaluation of the association of genetic profiles with clinical specimens is recommended.
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