High resolution melting (HRM) curve analysis for rapidly and accurate determination of Salmonella spp. with invA gene

Salmonellosis is an infectious and common disease between humans and animals that is caused by different strains of Salmonella. Progress in molecular diagnostic methods, has led to accurate and easy detection and characterization of food microbial agent. The purpose of this research was to use HRM technique to access more accurate and rapid diagnosis of Salmonella bacteria by means of invA gene. 

Material & Methods:

In this study, diagnosis of Salmonella was done by polymerase chain reaction and high resolution melting curve ( PCR-HRM) using the sequence on invasion A gene (invA) as a marker. In total 9 Salmonella reference strains using a specific primer pair of genes invA were used to detect Salmonella.  

The expected size of PCR amplified fragments of invA gene was determined as 284bp. All tested strains were able to show a Salmonella specific melting curve with high resolution at thermal interval of 87.8-87.9°C.

The results showed that HRM using specific primers of invA gene can be used as an accurate and reliable technique for diagnosis Genus of Salmonella.