A study on histological changes of testis in micetreated with intratesticular gentamicin

Message:
Article Type:
Research/Original Article (بدون رتبه معتبر)
Abstract:

Arrestment of spermatogenesis in the recipient is an important step in spermatogonial stem cell transplantation.Studies have shown toxic effects of gentamicin on proliferation and differentiation of bone marrow stem cells. Thepresent study was conducted to evaluate the effect of intratesticular administration of gentamicin on induction ofazospermia in mice. Twelve male mice were randomly assigned to three experimental groups: in control group,the mice received no treatment. In saline group, the mice received 0.02 ml saline intratesticularly. In gentamicingroup, the mice received 0.02 ml gentamicin (40 mg/ml) intratesticularly. Two months after treatment, the testeswere removed and their weight was recorded. Then, the specimens were fxed in 10% formalin solution, stainedand evaluated using light microscope. There was no difference among experimental groups in testicular weight andmortality (P> 0.05). Although germ cells were depleted in saline and gentamicin groups, spermatogenesis was notarrested in none of these groups based on histopathological examination. In conclusion, the results indicated thatintratesticular administration of gentamicin might not serve as an effective method for induction of azospermia inmice.

Language:
Persian
Published:
Veterinary clinical research journal, Volume:5 Issue: 3, 2014
Pages:
181 to 186
magiran.com/p2183647  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!