Evaluation of the Effect of Dopamine D2 Receptor Antagonist on the Spermatogenesis Process in Rats Under Physical or Psychological Stress and Their Offspring
Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background and Objectives

Stress disrupts the neurotransmitter release and reproduction. Dopamine (D) is one of the most important neurotransmitters, and its receptor (D2R) has been identified in testis. The use of effective drugs on reproductive system may affect the physiology of offspring whose parents are under treatment. This study aimed to investigate the effect of D2R inhibition on testicular tissue and the process of spermatogenesis in stressed rats and their offspring.

Methods

In total, 72 adult male Wistar rats weighing 190±10 g were divided into groups of saline, sulpiride (D2R antagonist) (4mg/kg, ip), physical or psychological stress, and physical or psychological stress receiving sulpiride. Physical or psychological stress was induced by a communication box. At the end of the 14-day treatment, each male rat mated with three female rats. The male offspring were raised under normal conditions until puberty. Subsequently, the indices of spermatogenesis (number of spermatogonia cells, spermatocytes, spermatids, sertoli cells), seminiferous tubules diameter, thickness of testicular capsule, and serum testosterone concentration were evaluated in male rats under treatment and their offspring. Data were analyzed in SPSS software through a one-way ANOVA and Duncan test.

Results

Stress induction and/or sulpiride administration led to a significant decrease in the number of spermatogenic cells (spermatogonia, spermatocytes, spermatids, and sertoli), morphometric indices (seminiferous tubules diameter and thickness of testicular capsule), and testosterone concentration in parents and offspring, compared to the corresponding control groups (P<0.05).

Conclusion

Stress caused a decrease in spermatogenesis in parents and offspring. Moreover, the sulpiride administration intensified the adverse effects of stress on spermatogenesis in both generations.

Language:
Persian
Published:
Qom University of Medical Sciences Journal, Volume:14 Issue: 6, 2020
Pages:
18 - 30
magiran.com/p2185052  
برخی از خدمات از جمله دانلود متن مقالات تنها به مشترکان مگیران ارایه می‌گردد. شما می‌توانید به یکی از روش‌های زیر مشترک شوید:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 700,000ريال می‌توانید 100 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • دسترسی به متن مقالات این پایگاه در قالب ارایه خدمات کتابخانه دیجیتال و با دریافت حق عضویت صورت می‌گیرد و مگیران بهایی برای هر مقاله تعیین نکرده و وجهی بابت آن دریافت نمی‌کند.
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.