A Phylogenetic Analysis of HC-Pro Protein in Iranian Isolates of Bean Yellow Mosaic Virus
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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Introduction

Bean Yellow Mosaic Virus (BYMV) belonging to Potyvirus genus and Potyviridae family has a worldwide distribution and a broad host range. In the present study, the phylogenetic relationships of eight virus isolates isolated from different geographical regions of Iran (East Azerbaijan, Ardabil, Ghazvin, Zanjan, Hamadan, Khuzestan, Fars, and Kerman) during the 2017 and 2018 growing seasons were compared with other isolates that were available in the GenBank.

Materials and methods

Plants leaves with viral symptoms were sampled from faba bean fields of different geographical regions of Iran and were transferred to the laboratory. Then, to detect the virus-infected samples, first, DAS-ELISA test was performed using specific antisera. Then, the total RNA of the infected samples was extracted and HC-pro region of selected isolates was amplified and sequenced. Sequence evaluation, phylogenetic relationships, protein structure analysis, and the recombination detection of HC-pro region of the selected isolates were performed using various software.

Results

The selected Iranian isolates were placed in two separate monophyletic groups in the reconstructed phylogenetic tree. Five isolates collected from East Azerbaijan, Ghazvin, Zanjan, Fars, and Kerman and two Australian and Japanese isolates, all isolated from faba bean, were clustered in a monophyletic group. The isolates collected from Ardabil, Hamadan, and Khuzestan, with two other Iranian isolates available in the GenBank, two Indian isolates, and two Japanese isolates were clustered in another group. According to the recombination detection analysis, none of the selected Iranian isolates has been detected as a recombinant isolate. 

Discussion and conclusion:

Understanding the genetic and recombinant changes of the viral population is an important prerequisite for the efficient diagnosis, effective management, and long-term disease control. The results are helpful in developing strategies that lead to BYMV resistance and may be effective in the future if the disease becomes widespread in line with adopting management strategies.

Language:
Persian
Published:
Biological Journal of Microorganism, Volume:9 Issue: 34, 2020
Pages:
55 to 70
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