Influence of Medium and Plant growth regulators on in vitro growth indexes of Tecomella undulata (Roxb.) Seem

Tecomella undulata is one of endemic and endangered plant species in Iran. It is belong to  Bignoniaceae family grown in southern of Iran. Due to the specific characteristics of this plant in various aspects of medicine, ornamentation and natural resources, its mass production through tissue culture and creation of identical plants seems necessary. Effects of growth regulators on plant growth indices such as number of buds and branches, shoot length, branch greenness and rooting were studied by bud explants under in vitro conditions. The optimal aseptic technique was obtained by immersing the buds in 0.1% mercuric chloride solution for 4 minutes in spring. The best shooting was observed in MS basal medium containing BA 1 treatment and K0.5 mg/L. This treatment was the best selection for this phase with the average shoot growth of 4.9 cm and shoot length of 7.78 cm. At the rooting stage, despite the numerous treatments of the medium and hormones (various concentrations of auxin hormone), the onset of rooting was only observed in 1/2 MS IBA2.