Phenotypic and genotypic evaluation of fluoroquinolones resistance in Klebsiella pneumoniae isolates from urinary tract infections in Bojnourd city

Urinary tract infection (UTI) is one of the most common bacterial infections, accounting for a significant proportion of hospital clients (about 30-40%). The emergence of resistance to cephalosporins, fluoroquinolones and trimethoprim-sulfamethoxazole which were often used to treat general and nosocomial infections, delayed effective treatment of these diseases and subsequently resulted in increased infections and mortality of patients.

In this study, 30 specimens of positive cultures of urinary tract infection which were referred to Imam Reza Hospital laboratory in Bojnourd were studied. The resistance and susceptibility of the isolates were determined by disc diffusion method. The presence of QnrA gen was assessed by molecular methods with specific primers.

The phenotypic resistance of isolates to nalidixic acid, levofloxacin and ciprofloxacin were 43.33%, 26.66% and 40%, respectively. Also, genotypic analysis of the isolates showed 6.66% of the studied genes.

Based on the results of the present study, it can be said that resistance to antibiotics belonging to the fluoroquinolones is not yet widely high.