Protective Effect of Bene (Pistacia Atlantica) on Busulfan-Induced Renal-Liver Injury in Laboratory Mice

Busulfan is a chemotherapy drug that has side effects such as hepatic and renal injury. Pistacia atlantica from the family Anacardiaceae has a potent antioxidant agent due to phenolic compounds. The purpose of this study was to investigate the effects of Pistacia atlantica on histopathology and liver and kidney function indicators in busulfan-induced liver and kidney injuries in adult mice.

A total of 28 BALB/c mice were randomly divided into four groups (control, busulfan, bene and bene+busulfan). The busulfan group received 10 mg/kg busulfan as a single dose and intraperitoneally. The bene group received plate containing 10% of bene for 35 days. The bene+busulfan group received 10 mg/kg busulfan+10% bene. Then, liver enzymes alanine amino transferase, aspartate amino transferase, urea, creatinine and histopathology of liver and kidney investigated.

The mean urea (P= 0.009) and creatinine level (P= 0.02) in the busulfan group was 55.2±4.23 and 0.8±0.11 mg/dl that it was significantly higher than the bene + busulfan. A significant decrease in ALT level in the bene + busulfan group compared to the busulfan treated group (P= 0.03). In the bene treated group steatosis, necrosis and fibrosis in the liver parenchyma and glomerular sclerosis, inflammation and tubular atrophy in kidney tissue, was not observed.

The results of present study indicated that administration of 10% bene for 35 days improved histopathology of kidney and liver as well as functional index of liver and kidney after renal-liver injury.