The Effect of Iron and Silver Nanoparticles on Biofilm Formation in Bacteria Isolated From Clinical Specimens

Introduction :

Nosocomial infections are a major global problem. Using metallic oxide nanoparticles to counter bacterial infections as an alternative to antibiotics can be effective. The aim of this study was to evaluate the effect of iron and silver nanoparticles on biofilm formation in bacteria isolated from clinical specimens and the possibility of using these nanoparticles on laboratory surfaces.

This study was performed on three bacterial strains isolated from different wards of Imam Reza, Imam Hossein and Taleghani hospitals in Kermanshah. Microbiological and molecular standard methods were identified and their antibiotic resistance was determined. The 96-well plate was used to investigate the disinfecting effect of silver and iron nanoparticles on biofilm formation. Data were analyzed by SPSS software.

Results showed 9 blood samples (30%), 5 skin samples (16.67%) and 16 urine samples (53.33%). The highest rate of inhibition of Escherichia coli biofilm formation by silver and iron nanoparticles was 93% and 64%, respectively. The inhibition of biofilm formation in S. epidermidis by silver and iron nanoparticles was 86 and 64%, respectively, and the highest inhibition of biofilm formation by Pseudomonas aeruginosa by silver and iron nanoparticles was 96 and 88%, respectively.

The use of silver and iron nanoparticles has a high potential to kill the bacteria in the biofilm of P.aeruginosa.