Application of Pectin- tagged Nano Silver and Triacontanol on In Vitro Microruberization of Solanum tuberosum L. cv. Agria

Nanotechnology is effective in the rapid detection of plant diseases, identifying and removing pesticide residues, increasing the shelf life, packaging and transporting in crops. The aims of this study were to evaluate the effect of pectin- tagged nano silver nanocomposite and triacontanol on in vitro contamination, micropropagation and microtuberization of potato under in vitro condition. For this purpose, a factorial experiment was conducted in a completely randomized design with four replications on potato explants of mentioned cultivar. In first experiment, the number of healthy explant without any bacterial and fungal symptoms were calculated after 1 week. In second experiment, four weeks after cultivation of explants, micropropagarion in microtuber number, microtuber length and weight, diameter of the tubers, the length of bud, the percentage of non-dormancy microtubers, the germination percentage and the rate of microtuberization were measured. Our result showed that application of 50 mg/L for 30 min pectin-tagged nano silver increased healthy explants more than 50%. Among the different concentration of triacontanol, using 0.5 mg/L was the best response for traits such as microtuber number, microtuber length and weight. Also, using 0.5 mg/L triocontanol was the best protection for all microtuber traits except microtuber weight. Although the experiment was performed without the use of growth regulators, the addition of graphene oxide nanoparticles to the medium improved the micropropagation and microtuberization performance. In general, the results showed that 0.5 mg/L was the best concentration for microtuberization. Microtubers production technology is used as a tool to reduce the time needed to produce plant biomass, increase seed tuber quality (reduce pathogens), and microtubers production throughout the year.