Evaluation of Diversity of Self-Incompatibility Locus and Isolation of the First Allele of this locus in Indigenous Quince (Cydonia oblonga Mill.) Genotypes of Iran

Quince (Cydonia oblonga Mill.) is considered as a self-compatible species, but some levels of self-incompatibility also have been reported in its cultivars and genotypes. Therefore in this research allelic diversity, isolation and deposition of alleles has been considered in some indigenous quince cultivars and genotypes of Iran. The primes were designed according to the C1 to C5 conserved regions of apple and pear species. 55 genotypes, cultivars and related species of quince were evaluated and amplification of this locus demonstrated various band lengths between 500 to 1100 bp with 13 various sizes. After partial sequencing, these bands primarily were coded as Sq1 to Sq13, but the frequency of two putative alleles, Sq13 and Sq4 where higher than other respectively with 17.7 and 14.4%. Sequencing of the first self-incompatibility allele (S1) in KM1 genotype or cultivar Torsh Esfahan revealed the highest homology of this allele with S6 of apple species Malus domestica Borkh. and Ss (S111) of pear species Pyrus communis L., respectively, with 94 and 93 of nucleotide similarity.