Role of miR-214 in Sensitization of Human Colorectal Cancer Cells to Doxorubicin by p53 Targeting

The upregulation of miR-214 is reported to reverse chemotherapeutic resistance or sensitivity in many human malignancies.

This study aimed to investigate the potential function of miR-214 in the apoptosis induction by targeting p53 in human colorectal cancer cells (CRC) in combination with doxorubicin (DOX).

miR-214 mimics were transfected to HT-29 CRC cells. Following that, DOXwas utilized to treat the transfected cells. additionally, apoptosis, migration, and cell viability were evaluated by flow cytometry, scratch-wound motility, and MTT assays, respectively. Furthermore,qRT-PCRwas employed to evaluatethe expression level of miR-214 and p53.

miR-214 transfection significantly inhibited the cell proliferation rate (P<0.05), harnessed migration (P<0.05), and induced apoptosis (P<0.05) in the HT-29 cells after 48 h. Furthermore, more effectiveness was observed in combination with DOX. Additionally, miR-214 transfection resulted in a reduction in p53 expression offering that might be a potential target for miR-214.

In conclusion, miR-214 sensitizes HT-29 cells to doxorubicin by targeting p53 indicating thesignificant roleof this miRNA in colorectal cancer chemotherapy.