Bioethanol Production from Bread Waste with Enzymatic Hydrolysis and Fermentation by Saccharomyces Cerevisiae
Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:

Bread waste is the common part of food biomass in the world. The aim of this study was to investigate the effects of saccharification time and also substrate concentration on the amount of glucose as well as bioethanol obtained from bread wastes folowing hydrolysis processing. The bread wastes were crushed to small parts and then mixed with water at ratio of 10-15 (w/v%). Alpha-amylase and glucoamylase enzymes were used for liquefaction and saccharification, respectively. The effects of saccharification time and substrate loading parameters on the amount of glucose were investigated by using response surface methodology (central composite design) with Design Expert software. The glucose-derived from hydrolysis processing was measured by glucose kit. Aflatoxin contents of the bread wastes (as control sample) and optimum sample obtained from the hydrolysis processing (sample with the maximum glucose) were measured. Fermentation processing was carried out by using Saccharomyces cerevisiae yeast. The results showed that the highest amount of glucose (100.21 g/l) in enzymatic hydrolysis was obtained at the saccharification time of 48 h and substrate loading of 150 g/l. The hydrolysis processing at the higher concentrations and longer duration, due to high viscosity and adhesion, reduced the concentration of glucose, so high concentration did not have any favorable effect on the hydrolysis processing. The hydrolysis processing reduced Aflatoxin B1 and B2 at a ratio of 76% and 16%, respectively. The greatest amount of bio-ethanol in the fermentation phase (45.35 g/l) was obtained at 36 hours with efficiency of 88.7%. It could be related to the consumption of glucose produced in the hydrolysis phase due to proper growth of the cell mass during fermentation phase in this duration. The recommended time for the fermentation processing is 36 hours.

Language:
Persian
Published:
Agricultural Research, Education and Extension Organization, Volume:21 Issue: 75, 2021
Pages:
47 to 64
magiran.com/p2250859  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!