Enrichment and Rapid Detection of Vibrio Cholerae from Water Using Non-Culture Method

In microbial contamination of food and water, identification of trace amounts of contaminating bacteria has always been an interest and concern for researchers. The most common way to remove of this problem is culture-based methods in order to increase and enrichment of bacteria samples, which extends the bacterial detection process to several hours or days. One of the smart strategies to solve this problem is concentrated of bacteria using physical methods. The aim of the present study was to enrich of Vibrio cholerae as the most important water polluting germs using filtration method and evaluation of its function by culture method and two detection methods ATP and PCR assay.

A certain concentration of V. Cholerae was added into a specified volume of sterile water, artificially. Then, the bacteria were extracted from the medium and filtered using 0.450µm separable filters. Finally, the performance of the pre- and post-filtration process was compared using bacterial cell culture (CFU), ATP and PCR assay with the specific primers for the ompW gene of V. cholerae.

The results of this work showed that this method shows high efficiency and recovery performance. So that, samples had no positive response before filtration in both methods, but after filtration in isolated and recovered samples, the presence of bacteria was detected in both ATP and PCR methods.

In conclusion, this strategy can detect V. cholerae in non-culture and in the least amount of time in contaminated water samples.