Investigation of genetic variation of forage yield, and clustering of some selected Phalaris aquatica L. genotypes by clonal evaluation

The objective of this study was to evaluate the genetic variation of 26 selected genotypes of Phalaris aquatica L. based on important agronomic traits. Each selected genotype was divided into four clones and they were space planted according to a randomized complete block design with four replications under irrigation conditions in the Khalatposhan research station of Tabriz University, Iran. Data collection was started in March 2017 for two years. The results of analysis of variance showed significant differences among the studied genotypes for most traits (P<0.01). Accordingly, the mean comparisons of the traits among genotypes were conducted. Using principal component analysis (PCA), the first two components were accounted 66% of the total variation. The traits like forage yield in both cuts, crown diameter, plant height, and seed yield had the most important role in determining the first component. Based on cluster analysis, the 26 genotypes were grouped into four distinct groups. Based on the mean comparison results, principal component, and cluster analyses, 12 genotypes with the highest forage and seed yields in both years were selected as superior genotypes. Therefore, this population provided a suitable genetic source for breeding improved varieties using polycross progeny tests and breeding improved synthetic variety.