The effect of L-arginine on estrogen level and ovarian cyst in aged rats with polycystic ovaries

Article Type:
Research/Original Article (دارای رتبه معتبر)
Background and Objective

Menopause in women is associated with many complications that most of them are related to the decrease of estrogen levels in this period. Treatment with high doses of estrogen is common but has side effects. In this study, the effect of l-arginine administration on the level of this hormone in elderly rats was investigated.

Materials and Methods

Elderly Wistar rats were first studied with the help of Papanicolaou test to identify the stage of female sexual cycle. If confirmed to have diestrus phase, the rats were randomly classified into the following groups: control (saline 1 ml/kg, i.p.) and l-arginine group (5, 25, and 50 mg/kg). They were injected saline or l-arginine over a period of three to nine days. At the end, the rats were anesthetized by an i.p. injection of ketamine 100 mg/kg and xylazine 20 mg/kg and the blood samples were collected and the estrogen levels were measured with ELISA kit. The rats’ ovaries and uteri were also biometrically examined and fixed in the formalin. They were stained by H&E method and the number of cysts in the ovaries were counted. Data were analyzed by the ANOVA.


L-arginine at all doses (5-50 mg/kg) during all injection periods from three to nine days significantly increased the estrogen levels, but prominently reduced the ovarian cysts at the lowest dose (5 mg/kg).


Low doses of l-arginine over short periods of time can relieve menopausal problems including estrogen levels and ovarian status, probably by the modulator nitric oxide.

Journal of Basic & Clinical Pathophysiology, Volume:9 Issue: 1, Winter-Spring 2021
41 to 46  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!