Identification and Toxinogenesis of Aspergillus spp. fungus on poultry diet in Khorramabad

Fungal toxins are responsible for many human and animal diseases and epidemics. This study was performed to identify and investigate the toxicogenic isolates of Aspergillus species in 30 poultry farms in Khorramabad. The 180 samples of poultry diets were collected and transferred to the laboratory. The samples were isolated and purified using different culture media such as Potato Dextrose Agar (PDA) and Yeast Extract Agar (YEA). Then the isolates were identified using valid mycological keys and articles. The toxinogenesis of this fungus was investigated using coconut agar medium, thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) on corn and peanut media. The results showed that 38 isolates were identified in the poultry diet. The corn with 20 isolates (66.6%) and finisher with three isolates (10%) had the highest and lowest frequencies, respectively. Also, by using coconut agar and TLC toxinogenesis, some of the isolates of this fungus have been proved and in HPLC experiments it was determined that this fungus has the ability to produce all four types of aflatoxins B1, B2, G1 and G2. So due to the presence of different toxinogenic isolates of this fungus on the diet and their pathogenicity, compliance with international standards of maintenance diets is recommended.