Investigation of the Effects of 6 Weeks High Interval Intensive Training Period on the Expression of the HDAC4, HDAC5, Dch2 and Myogenin Gens in the Plantaris Muscle of the Aged Male Wistar Rats

One the major reason of the age-related muscular atrophy, Sarcopenia, is neurogenic atrophy. Despite this issue, the effects of some pathway which have probability effect, such as: kind of training methods, especially high interval intensive training, on the main factors of the neurogenic cascades is not yet clear. Therefore, the aim of this study was to investigate the effects of high interval intensive training period on the expression of the HDAC4, HDAC5, Dch2 and Myogenin gens in the plantaris muscle of the aged male Wistar rats. 28 male Wistar rats randomly assigned in to four groups (N=7, adult control, N=7, adult training, N=7, aged control, N=7, aged training). The training group performed HIT 5 times per week for 6 weeks. 48 hours after the end of the last session of training with control group were anesthetized and sacrificed. Then the plantaris muscle was removed. Real time PCR method was used to determine the gene expression levels of HDAC4, HDAC5, Dch2 and Myogenin. For the statical analyses two-way ANOVA and Tukey were used. The statical significant assumed P≤0.05. The results of this study have been shown that HDAC4, HDAC5 and Myogenin significantly decreased. The amount of the Dch2 increased significantly compared to young control group. According to the results of this study, it seems high interval intensive training suppress the neurogenic atrophy   in the orders adult.