The effect of Endurance Training on Oxidative Stress Induced by Ischemia in Male Rat Heart

Inroduction & Objective:

Regular exercise reduces the incidence of cardiovascular diseases. In this context, choosing the type and intensity of training as a suitable model for further effectiveness is essential. The aim of this study was to investigate the effect of 8 weeks of endurance training on oxidative stress induced by ischemia in male rats.

In this study, thirty two male Wistar rats in the weight range of 220± 20g were used. After one week of adaptation to the new environment, mice were randomly divided into four groups (n=8): 1- Control group, 2- Isoproterenol group (ISO), 3- Endurance exercise group (EnEx) 4- Endurance exercise group + Isoproterenol (EnEx + ISO). EnEx and EnEx + ISO groups were undergoing endurance training for 8 weeks. At the end of the eighth week of training, Isoproterenol (85 mg / kg) was administered intraperitoneally for 2 consecutive days. Then, the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) and malondialdehyde (MDA) were measured with special kits.

Results showed that cardiac ischemia significantly decreased antioxidant enzymes and increased lipid peroxidation (MDA) as compared to control and exercise groups, while eight weeks of endurance training before ischemia increased antioxidant enzymes activity and decreased the level of MDA in comparison to ischemia group.

The results of this study showed that endurance training can have a therapeutic effect against ischemia-related injuries by reducing oxidative stress and diminish ischemic heart disorders.