Antibacterial Effects of Ethanolic Extracts of Ziziphus jujuba, Medicago sativa, Reum ribes and Hyssopus officinalis on Some Standard Gram-Positive and Gram- Negative Bacteria in Vitro

Today, with increasing resistance to synthetic drugs, the demand for natural and safe alternatives to these drugs increases. Therefore, the aim of this study was to determine the antibacterial effect of ethanolic extracts of Ziziphus Jujuba, Medicago sativa, Reum ribes and Hyssopus officinalis on some standard bacteria.

This experimental study was performed in the Microbiology Laboratory of Islamic Azad University, Ahar Branch. Plants were identified by botanists after being collected from natural habitats. Extraction was performed by Soxhlet extractor and the antimicrobial effects of the extracts were determined by agar well diffusion and determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) on standard bacteria in vitro.

Z. Jujuba and M. sativa plant extracts have been effective on gram-negative bacteria and extracts of R. ribes and H. officinalis plants have been effective on gram-positive bacteria, with increasing the concentration of extracts, the antibacterial property increases significantly. MBC / MIC results showed that Z. Jujuba and M. sativa plant extracts were most susceptible to P. aeruginosa and E. coli and R. ribes and H. officinalis extracts were most susceptible to S. aureus and B. cereus.

Z. Jujuba, M. sativa, R. ribes and H. officinalis plants had significant antibacterial effects, but to introduce these plants as antibacterial drugs requires more complete and comprehensive studies in vivo.