THE FREQUENCY OF RS877899 POLYMORPHISMS IN THE SEPP1 GENES AND TYPE 2 DIABETES, ITS RELATION TO BLOOD GLUCOSE LEVELS AND BLOOD SELENOPROTEINE P AND OXIDATIVE STRESS INDICES

Ala234Thr single nucleotide polymorphism can affect the level of oxidative stress capacity and serum selenium levels. On the other hand, blood glucose and insulin levels regulate the expression of SEPP1 gene. It is hypothesized that this polymorphism may be significantly associated with diabetes. The aim of this study was to determine the genotype of Ala234Thr polymorphism in patients with type 2 diabetes and its effect on glucose levels, selenoprotein p levels, and oxidative stress status of patients.

In this study, biochemical indices (FBS & HbA1C), and serum oxidative stress indices (MDA and total antioxidant capacity) were measured in both patient and healthy groups, and Tetra-ARMS PCR was used to determine the genotype of A234T polymorphism.

There was no significant relationship between demographic indicators (age and gender) with the incidence of type 2 diabetes, but there was a significant relationship between biochemical indicators and oxidative stress (p = 0.001). Genotyping results showed that the frequency of GG genotype in the control group was higher than the patient group, but it was not statistically significant. It was also found that the risk of type 2 diabetes in people with GA genotype is 2.14 times higher than others (p = 0.035).

The results demonstrated the effect of high levels of SEPP1 on the pathogenesis of type 2 diabetes through oxidative stress pathways. Rs3877899 polymorphism is associated with an increased risk of type 2 diabetes in the subjects.