Investigated the effects of ultrasound waves on serum levels of DHEA-SO4, testosterone, and testicular tissue in male rats

Many studies have shown the relationship between ultrasound and the function of the reproductive system. However, the mechanism of ultrasound waves action on sex hormones and testicular tissue is not perfectly clear. Accordingly, the present study investigated the effects of ultrasound waves on serum levels of DHEA-SO4, testosterone and testicular tissue in male rats.

Male wistar rats were divided into three groups: control group which was not exposed to ultrasound waves, rats that were exposed to ultrasound waves for 1h/day, and rats that were exposed to ultrasound waves for 6h/day. The serum levels of testosterone and DHEA-SO4 were measured using ELISA. The testicular tissue slides were prepared and analyzed microscopically.Data were analyzed using one-way ANOVA.

Serum levels of testosterone and DHEA-SO4 and spermatogonia, spermatocytes, sertoli cell count and seminiferous tubules morphology did not significantly changed in groups exposed to ultrasound waves compared to control animals.

Our findings show that ultrasound waves do not significantly influence male reproductive system function and histology.