A Review of Analytical Methods for the Measurments of Clopidogrel Active Metabolite in Biological Matrices

Despite the widespread use of clopidogrel as a prodrug in a wide range of patients, the researchers found that the drug couldn't have sufficient antiplatelet effects at a usual dose in three to forty percent of patients. So on, measuring clopidogrel active metabolite concentration is seems necessary to evaluate the effects of this drug in each patient.

Due to the importance of determining clopidogrel active metabolite plasma level, in this article, we reviewed and compared all the analysis methods for that which could affects on correct pharmacokinetic evaluation of clopidogrel and thus play an important role in prescribing the appropriate dose of that in each individual therapy. Despite numerous reports of the clopidogrel analysis method, only thirteen studies have been published to analyzing the concentration of clopidogrel active metabolite in a biological matrices. As a result of the very low concentration and short half-life of the clopidogrel active metabolite in the biological matrice, the method used for determining itʹs concentration profile should be able to measure values in picogram per millilitre scale.

According to the complete summary of the analyzing methods, employing advanced device analysis systems such as UHPLC lead to much more accurate separation and quantification of analytes. Also, employing the MS / MS detector adding the possibility of measuring the clopidogrel plasma level in the same matrices.