Extraction of titanium nanoparticles of Saturaja rechengri Jamzad us-ing ultrasound techniques; the antimicrobial effect against food-borne pathogens and its cytotoxicity against normal and cancer cell lines

Green synthesis of nanoparticles has some advantages including the commitments  to avoid  harmful chemicals, clean process, eco-friendly, easy preparation, economical and control on size and shape of particles. In this study, water extract of Saturaja rechengri Jamzad was prepared and titanium nanoparticles  produced using green and ultrasound techniques. Then, in order to characterize the nanoparticles, UV analysis, energy dispersive x-ray spectroscopy and TEM and SEM analysis were performed. MIC and MBC of nanoparticles against E. coli O157:H7 and L. monocytogenes and cytotoxicity in 12.5, 25, 50, 100 and 200 mg/ml on HT-29 and HEK-293 cell lines were also determined. Nanoparticles had absorption at 352 nm which showed synthesis of titanium nanoparticles using Saturaja rechengri Jamzad extract. Spherical shape of nanoparticles was detected in colloidal solution with 37.4 to 49.6 nm. Titanium nanoparticles could stop the growth of E. coli O157:H7 and L. monocytogenes at 20 µg/ml. The nanoparticles did not revealany cytotoxic effect on the  normal cells, while it was  stop the growth of 50% of cancer cells at 183 µg/ml. The results of this study decreased the anxiety of using titanium nanoparticles and increased the potential  of using this nanoparticle in food packaging  to finally extend shelf life of food.