The culture of organization is a system of common means and concepts that differentiate one organization from the other organizations. The role of human capital is a great importance in creating and developing organizational culture. The aim of current study is to identify strategies of the change of organizational culture with emphasis on human capital in cultural organizations. The researcher considers strategies of  human capital that change organizational culture in cultural institutions particularly Ministry of Culture and Islamic Guidance. The Methodology of the research is descriptive-analytical and data collection tool is Likert scale questionnaire comprising 4 factors and 24 items .The research statistical population concludes 4000 employees that out of them a sample of 384 was randomly selected, based on Morgan Table.  the results of the research indicated that outstanding performance management strategies, talent management, organizational training and learning, and payment and benefits have a positive impact on changing organizational culture of  the Ministry of Culture and Islamic Guidance and can be effective in changing the current situation to the desired state of organizational culture.